Research articles
ScienceAsia 49 (2023):ID 529-540 |doi:
10.2306/scienceasia1513-1874.2023.043
Optimization of phenolic extraction from Syzygium
samarangense fruit and its protective properties against
glucotoxicity-induced pancreatic β-cell death
Kanchana Suksria,c, Bancha Yingngamb, Nipaporn Muangchanc,*
ABSTRACT: Long-term exposure to high glucose levels causes glucotoxicity in pancreatic ?-cells associated with
diabetes mellitus. The presence of natural antioxidant phenolic compounds, including the compounds from Syzygium samarangense, may help mitigate this metabolic disorder. This study aimed to optimize microwave-assisted
extraction (MAE) for maximum phenolic recovery and assess the protective properties of Syzygium samarangense
extract (SSE) against pancreatic ?-cell mortality caused by glucotoxicity. We demonstrated that the MAE-based
response surface methodology provided higher concentrations of total phenolics than the conventional reflux method
(10.21?0.22 mg GAE/g and 6.44?0.13 mg GAE/g, respectively). According to in vitro studies, the percentage of
viable rat insulinoma cell lines (INS-1 cells) was significantly lower when cultured in high glucose (HG) medium
(40 mM) than in normal glucose medium (11.1 mM). Upon treatment of INS-1 cells with SSE (10, 50, and 100 ?g/ml)
in combination with HG, SSE exhibited a protective effect on the cell viability and inhibited cell apoptosis. In addition,
SSE reduced intracellular superoxide ion concentrations in a dose-dependent manner. We conclude that the phenolics
of SSE could function as antioxidants, thereby protecting pancreatic ?-cells against glucotoxicity-induced apoptosis.
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| a |
Division of Pharmacology and Biopharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Burapha University,
Chonburi 20131 Thailand |
| b |
Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmaceutical Sciences, Ubon Ratchathani
University, Ubon Ratchathani 34190 Thailand |
| c |
Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University,
Ubon Ratchathani 34190 Thailand |
* Corresponding author, E-mail: nipaporn.m@ubu.ac.th
Received 5 Jun 2022, Accepted 21 Mar 2023
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