Research articles
ScienceAsia 49 (2023):ID 403-410 |doi:
10.2306/scienceasia1513-1874.2023.029
In vitro anticancer efficacy of silibinin-loaded PLGA
nanofibers against A549 non-small-cell lung cancer cells
Qiong Wu, Shiguang Xu, Wei Xu, Hao Meng*
ABSTRACT: In recent years, amount of evidence suggests that silibinin (SLB), an active constituent isolated from the
seeds of Silybum marianum, possesses pleiotropic anticancer effects in different cancers. In this study, poly (lactic-coglycolic acid) (PLGA) polymer was used to fabricate SLB containing nanofibers (NFs) by the electrospinning method.
The NFs were characterized by their unique morphological features, degradation rate, and drug release profile. The
cytotoxic effects, production of intracellular reactive oxygen species (ROS), and the gene expression of apoptotic
proteins were investigated in A549 human lung adenocarcinoma cells and normal human lung epithelial cell line
(BEAS-2B cells) treated with free SLB and SLB-loaded PLGA NFs. The results indicated that both free SLB and SLBloaded NFs had no effect on BEAS-2B cells, but were cytotoxic against A549 cells, increasing intracellular ROS levels
in cancer cells. In addition, both agents induced the gene expression of apoptotic proteins, and the SLB-loaded NFs
acted more efficiently than the free SLB. With further study, hence, the SLB-loaded NFs could be potentially used as an
anti-cancer agent against human lung adenocarcinoma.
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Department of Thoracic Surgery, General Hospital of Northern Theater Command, Shenyang 110011 China |
* Corresponding author, E-mail: mh7713642@sina.com
Received 26 Apr 2022, Accepted 11 Dec 2022
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