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Research articles

ScienceAsia 48 (2022):ID 827-832 |doi: 10.2306/scienceasia1513-1874.2022.123

Effect of diosgenin in the inhibition of gastric cancer cell proliferation and its mechanism in combination with TRAIL to induce apoptosis

Qingwei Zhanga,†, Sheng Zhengb,†, Xuelian Baic, Chuntian Mad, Yuqian Qie,*

ABSTRACT:      Clinical observations suggest that identifying novel curative and preventive approaches by targeting the tumor cells without affecting the normal cells is appreciable. Diosgenin (DG) is a natural sapogenin that has been reported of having pro-apoptotic and anti-cancer properties against various neoplasia. Hence, the present study investigated the effect of combined DG/TNF-related apoptosis-inducing ligand (TRAIL) on gastric cancer cell lines (BGC-823) in vitro. Cell viability and IC50 for DG and TRAIL alone or in combination were determined using MTT. The apoptosis rate was assessed by ELISA cell death assay and Caspase 8 activity assays. The gene expression evaluation of candidate genes, including survivin, Bcl-2, XIAP, c-IAP1, c-IAP2, and c-FLIP, were accomplished before and after the treatment by quantitative real-time PCR. Our results demonstrated that DG synergistically enhanced the cytotoxic effects of TRAIL (p < 0.01). DG could exaggerate cell apoptosis through TRAIL-induced apoptosis and amplify the Caspase 8 activity. These results were confirmed by decreasing anti-apoptotic genes? expression levels. Overall, our findings shed light on a novel strategy of TRAIL-induced apoptosis in combination with DG for the treatment of gastric cancer.

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Received 16 Dec 2021, Accepted 10 Mar 2022