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Research articles

ScienceAsia 48 (2022):ID 532-537 |doi: 10.2306/scienceasia1513-1874.2022.075

Hedyotis diffusa Willd extract protects rat renal tubular epithelial cells from high glucose-induced injury by inhibiting PI3K/AKT signaling pathway

Haiping Donga, Qing Zhangb,*

ABSTRACT:     : The effects of Hedyotis diffusa Willd on high glucose (HG)-induced injury of rat renal tubular epithelial cells and its related regulatory mechanisms were investigated. In this study, NRK-52E cells were cultured in a normal glucose concentration (5.5 mM glucose) or HG concentration medium (30 mM glucose), and treated with the concentration gradient of H. diffusa extract (50, 100, 200 ?g/ml). The results suggested that H. diffusa extract has a protective effect on the cell viability of NRK-52E cells and inhibited the HG-induced oxidative stress through the detection of malondialdehyde content and specific activities of superoxide dismutase and glutathione peroxidase. The extract also inhibited the HG-induced apoptosis. In addition, the epithelial-to-mesenchymal transition (EMT) indicators including alpha-smooth muscle actin (?-SMA), E-cadherin, N-cadherin and vimentin were measured, it was found that the HGinduced EMT of NRK-52E cells was inhibited by the extract. Moreover, H. diffusa extract exerted an inhibitory effect on the PI3K/AKT signaling activation induced by HG. Hence, H. diffusa extract could protect rat renal tubular epithelial cells from HG-induced injury by inhibiting PI3K/AKT signaling pathway.

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a Department of Endocrinology, Yueqing People?s Hospital, Wenzhou, Zhejiang 325600 China
b Department of Endocrinology, Chongqing Qianjiang Central Hospital, Chongqing 409099 China

* Corresponding author, E-mail:

Received 2 Nov 2021, Accepted 24 Feb 2022