Research articles
ScienceAsia 48 (2022):ID 151-158 |doi:
10.2306/scienceasia1513-1874.2022.021
Chang-Kang-Fang ameliorates irritable bowel syndrome with
diarrhea in rat by regulating gut microbiota and improving
intestinal barrier
Min Lua,b,?, Xinyu Fanc,d,?, Yuanyuan Zhenge, Weiqian Konge, Ruyi Jie, Hui Xiee,*
ABSTRACT: Chang-Kang-Fang formula (CKF), a multi-herb traditional Chinese medicinal formula, has been clinically
used for treatment of irritable bowel syndrome with diarrhea (IBS-D). Though we have reported the compounds of
CKF and their therapeutic effect on IBS-D rats, the exact underlying mechanism is still not clear. The aim of this study
was to clearly define the effect of CKF on IBS-D by regulating gut microbiota and promoting intestinal barrier. IBS-D
rats were established by psychosocial stress (restraint) combined with the peripheral stimulation (senna leaf gavage)
stress. The changes of body weight and the number of fecal pellets were investigated during the experiment. The
effect on intestinal sensitivity was assessed based on the abdominal withdrawal reflex (AWR) scores and the intestinal
permeability, the expression of zona occludens 1 (ZO-1), measured by immunohistochemistry. The composition of gut
microbiota was detected through 16S rRNA. The administration of CKF significantly had therapeutic effects on IBS-D
rats based on the decreased AWR scores and the increased number of pellets. However, the changes of body weight were
not observed. In addition, CKF could up-regulate the expression of ZO-1 in the colon and rebalance the gut microbiota
of IBS-D by increasing the abundance of Lactobacillus, Allobaculum, Roseburia, and Lachnospiraceae_NK4A136. CKF
potentially alleviated IBS-D through regulating gut microbiota and improving the intestinal barrier function.
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| a |
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese
Medicine, Nanjing 210023 China |
| b |
Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210023 China |
| c |
Nanjing Lishui District Hospital of Traditional Chinese Medicine, Nanjing 210023 China |
| d |
Clinical College of Jiangsu Health Vocational College, Nanjing 210023 China |
| e |
The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023 China |
* Corresponding author, E-mail: xiehuitcm@163.com
Received 21 Apr 2021, Accepted 13 Oct 2021
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