Research articles
ScienceAsia 47 (2021):ID 410-417 |doi:
10.2306/scienceasia1513-1874.2021.052
The protective effects of glycyrrhizic acid on heavy
metal ions-induced reproductive toxicity in Chinese
hamster ovary cells
Liang Gaoa, Pai Liub, Tianqi Dua, Jian Yinb,c,*, Hong Lia,*
ABSTRACT: In this work, Chinese hamster ovary (CHO) cells were used to examine the protective effects of glycyrrhizic
acid (GA) on the reproductive toxicity of heavy metal ions, including Cd2+ and Cu2+. As a result, both metal ions
induced significant toxicity in CHO cells after 48-h treatment, as revealed by a severe decrease in cell viability. GA and
glutathione (GSH) largely reduced the toxicity caused by Cd2+ and Cu2+, which could be due to their recovery of GSH
levels and superoxide dismutase (SOD) activities in CHO cells. In addition, GA and GSH significantly up-regulated the
gene expressions of glutathione S-transferase (gst), sod, and heme oxygenase (ho)-1, which could be another explanation
for their protective effects. More importantly, GA exhibited comparable protective effects as GSH but at much lower
concentrations (50?100 ?M v.s. 500?1000 ?M). Therefore, GA could be effective for the alleviation of reproductive
toxicity of Cd2+/Cu2+, which needs further investigation in animal models.
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| a |
Center of Reproduction and Genetics, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou
Municipal Hospital, Suzhou 215002 China |
| b |
CAS Key Laboratory of Biomedical Diagnostics,
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science, Suzhou
215163 China |
| c |
Jinan Guo Ke Medical Technology Development Co., Ltd, Jinan 250100 China |
* Corresponding author, E-mail: yinj@sibet.ac.cn, hongliszivf@163.com
Received 10 Aug 2020, Accepted 26 Apr 2021
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