Research articles
ScienceAsia 46 (2020):ID 133-141 |doi:
10.2306/scienceasia1513-1874.2020.019
Low-expression of microRNA-203a in mid-gestational
human fetal keratinocytes contributes to cutaneous
scarless wound healing by targeting Tenascin-C
Meng Chena, Feng Zhaoa, Zhe Wangb, Hongxin Langa, Kun Zhanga, Tao Zhanga, Rui Wanga,
Ping Shic, Xining Panga,c,*
ABSTRACT: This study aimed to detect the roles of miRNA-203a in fetal cutaneous regeneration. The levels of miR-203a in eight mid-gestational and ten late-gestational fetal epidermis tissues were detected by real-time qRT-PCR. The expressions of α-SMA, TGF-β2 and TGF-β3 were detected by real-time qRT-PCR and Western blot. Moreover, the proliferation and migration abilities of keratinocytes (KCs) and fibroblasts (FBs) were examined by using MTS and transwell cell migration assays. Bioinformatics analyses, luciferase reporter and ELISA assays were used to examine the potential target genes. The results showed that miR-203a was dramatically up-regulated in late-gestational fetal epidermis tissues. MiR-203a inhibited the migration and proliferation abilities of KCs and FBs. Tenascin-C (TNC) was a direct target of miR-203a and miR-203a regulated TGF-β isoforms and α-SMA. It was concluded that the low-expression of miRNA-203a promoted KCs and FBs migration by targeting TNC and regulating TGF-β isoforms and α-SMA.
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| a |
Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public
Health and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University,
Shenyang, Liaoning, China |
| b |
Department of Pathology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China |
| c |
Shenyang Amnion Bioengineering Technology R & D Center, Shenyang, Liaoning, China |
* Corresponding author, E-mail: pangxining@126.com
Received 10 Jul 2019, Accepted 22 Feb 2020
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