Research articles
ScienceAsia (): 245-252 |doi:
10.2306/scienceasia1513-1874...245
The contents of bibenzyl derivatives, flavonoids and a
phenanthrene in selected Dendrobium spp. and the
correlation with their antioxidant activity
Rattanathorn Choononga,b, Witcha Sermpradita,b, Tharita Kitisripanyac, Boonchoo Sritularakd,
Waraporn Putaluna,b,*
ABSTRACT: Dendrobium spp. (the Orchidaceae family) is widely distributed in Asian countries. The fresh or dried
stems of Dendrobium spp. have long been used in traditional Chinese medicine for their tonic and antipyretic properties.
Bibenzyl derivatives (moscatilin, gigantol, crepidatin, and chrysotoxin), flavonoids (eriodictyol and homoeriodictyol)
and phenanthrene lusianthridin are the major active compounds in the stems of Dendrobium spp. Most of these
compounds have been recorded in these plants in Thailand; however, their concentrations have not yet been reported.
Therefore, in this study, dried stems of selected Dendrobium spp. (33 species) in Thailand were extracted and analyzed
by using high-performance liquid chromatography (HPLC). The HPLC method was performed on a reversed-phase
column using a mobile phase gradient system of 20-32% acetonitrile in 1.5% acetic acid with a flow rate of 0.8 ml/min
and a detection wavelength of 280 nm. The HPLC method displayed good linearity with coefficients of determination
(R2 > 0.997) for the seven active compounds analyzed. The variation in intra- and inter-day analysis (%RSD) was less
than 0.5%. Additionally, the extracts of selected Dendrobium spp. were investigated for antioxidant activity by DPPH
and FRAP radical scavenging assays. The results showed good activities in some species. The correlation between the
seven active compounds and the antioxidant levels (IC50 and FRAP values) were significant in bibenzyl derivatives and
homoeriodictyol as determined by the Pearson correlation. In conclusion, the developed HPLC method can be used for
the determination of active compounds in Dendrobium spp., which is useful for further studies of the species containing
the highest levels of these compounds.
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| a |
Faculty of Pharmaceutical Sciences, KhonKaen University, KhonKaen 40002 Thailand |
| b |
Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology
(PANPB), National Research University-KhonKaen University, KhonKaen 40002 Thailand |
| c |
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400 Thailand |
| d |
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences,
Chulalongkorn University, Bangkok 10330 Thailand |
* Corresponding author, E-mail: waraporn@kku.ac.th
Received 11 Dec 2018, Accepted 30 Jun 2019
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