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Efficacy of Citrus hystrix sprays in decontaminating Streptococcus mutans on childrens toothbrushes

Kemthong Mitrakul^a,*, Ratchapin L. Srisatjaluk^b, Vimol Srisukh^c, Kadkao Vongsawan^a

 
ABSTRACT:     Toothbrushes are sometimes contaminated by micro-organisms that can cause infection. This study evaluates the efficacy of Citrus hystrix sprays compared with 0.12% chlorhexidine gluconate (CHX) for disinfecting bacteria on children's toothbrushes. The participants were 61 children with a high caries risk aged 8–11. Each subject received two toothbrushes to be used continually for 7 consecutive days. The first set of toothbrushes was used as a baseline without any disinfectant. The others were divided into 5 groups and sprayed with distilled water (control), 0.12% CHX, 6% makrut oil (MO), 10% MO, or 13% MO. Toothbrushes were placed vertically into sterile test tubes containing 8 ml of TYS20B medium and incubated overnight. Then we performed 10-fold serial dilutions from 10⁻¹–10⁻⁵, and spread them on BHI or MS agars for investigating facultative bacteria, oral streptococci, and Streptococcus mutans. Results showed that for total facultative bacteria, 10% MO and 13% MO gave the highest reduction rate (100%)>CHX (88%)>6% MO (81%). For S. mutans, 10% MO and 13% MO also showed the highest reduction rate (100%)>6% MO (90%)>CHX (88%). For oral streptococci, 13% MO gave the highest reduction rate (100%)>10% MO (91%)>CHX (88%)>6% MO (69%). There was no significant difference between oral sprays and 0.12% CHX in the reduction of total facultative bacteria, oral streptococci, and S. mutans (p>0.05).

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* Corresponding author, E-mail: mkemthong@yahoo.com

Received 17 Apr 2014, Accepted 6 Feb 2015