Research articles
ScienceAsia (): 393-399 |doi:
10.2306/scienceasia1513-1874...393
Molluscicidal activity of Camellia oleifera seed meal
Supunsa Kijprayoona, Vasana Toliengb, Amorn Petsoma, Chanya Chaicharoenpongb,*
ABSTRACT: A mixture of molluscicidal saponin compounds was isolated from a methanolic extract of seed meal of Camellia oleifera and tested against Pomacea canaliculata. The most potent saponin fraction showed an LC50 value of 0.66 ppm. This was then used as a marker for quantitative analysis of active molluscicidal compounds in commercial oil-seed camellia meals on HPLC fractionation. The active saponin content was found to be 0.25–1.26% w/w. Methanol was the preferred extraction solvent for analysis of saponin compounds from oil-seed camellia meal. The effect of oil-seed camellia meal on P. canaliculata in a field experiment was determined for three doses: 12.50, 15.63, and 18.75 kg/ha in terms of numbers of dead snails. After one day, all treatments containing oil-seed camellia meal killed 100% of the snails in the sample compared with just 3.8% in the control without any chemical additive. No rice plant damage was detected from any treatments with oil-seed camellia meal, and the dry grain yield was comparable to that of niclosamide treatment. Thus oil-seed camellia meals may be a useful molluscicide for organic rice production.
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Research Centre for Bioorganic Chemistry, Department of Chemistry, Faculty of Sciences, Chulalongkorn University, Bangkok 10330 Thailand |
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Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330 Thailand |
* Corresponding author, E-mail: chanya.c@chula.ac.th
Received 6 Jul 2013, Accepted 11 Dec 2014
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