Short reports
ScienceAsia (): 170-173 |doi:
10.2306/scienceasia1513-1874...170
Single-nucleotide primer extension assay of mtDNA to authenticate cattle and buffalo meat
Rohita Guptaa,*, D.N. Rankb, C.G. Joshib
ABSTRACT: A reliable method for identifying and differentiating cattle and water buffalo meat by fluorescence-based single-nucleotide primer extension assay is presented. The method used genotyping (single nucleotide primer extension) of the cyt b gene region of mitochondrial DNA extracted from meat samples. Meat samples were subjected to different levels of autolysis to simulate various meat processing technologies. The assay indicates absolute specificity and a high sensitivity, even to low DNA concentrations of 0.01 pg (1%) of adulteration in cattle-buffalo meat mixture, and can be applied with equal efficiency to fresh, cooked, or putrefied meat.
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| a |
Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India |
| b |
College of Veterinary Science, Anand Agricultural University, Anand- 388001, India |
* Corresponding author, E-mail: rohitagupta2001@gmail.com
Received 6 Nov 2010, Accepted 18 May 2011
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