Research articles
ScienceAsia 49 (2023):ID 595-602 |doi:
10.2306/scienceasia1513-1874.2023.058
A novel long noncoding RNA, AC005062.1, acts as an
oncogene in colorectal cancer cell by regulating MACC1
Zhixiang Li*, Qiang Xie, Binbin Wang, Dajun Yu, Chunfang Liu, Kai Wang, Ligong Zhang, Chao Zhu
ABSTRACT: A novel long noncoding RNA (lncRNA), AC005062.1, has shown an abnormal expression in bladder cancer
and small cell lung cancer; however, it has never been studied in colorectal cancer (CRC). In this study, we explored the
expression and role of AC005062.1 in CRC. The expression of AC005062.1 in CRC tissues and cell lines was determined
using quantitative real-time PCR (qRT-PCR). In vitro experiments such as CCK-8 assay, flow cytometry, wound scratch
test, and transwell assay were performed in HT-29 cells after knockdown of AC005062.1. Our results showed that
AC005062.1 was significantly up-regulated in CRC tissues and positively correlated with advanced clinical stage, larger
tumor size, lymphatic metastasis, distant metastasis, and vascular invasion. Knockdown of AC005062.1 in HT-29 cells
significantly inhibited cell proliferation, migration, and invasion and promoted cell apoptosis and G1 arrest. mRNA and
protein expression levels of Metastasis Associated in Colon Cancer 1 (MACC1), a neighboring gene of AC005062.1, were
decreased after the knockdown of AC005062.1. Our study demonstrated that AC005062.1, which acts as an oncogene,
may be involved in CRC tumorigenesis and progression by regulating MACC1.
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Department of Tumor Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004 China |
* Corresponding author, E-mail: lizhixiang.118@163.com
Received 2 Dec 2022, Accepted 8 May 2023
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