Research articles
ScienceAsia 49 (2023): 290-296 |doi:
10.2306/scienceasia1513-1874.2023.016
Identification of key odor compounds in the burnt smell of
upper tobacco leaves through the molecular sensory science
technique
Ruili Lia, Kang Chena, Liqun Lib,*, Saiyue Zhaob, Chunsheng Guob, Xinhui Wanga, Junsong Zhanga, Miao Lianga,*
ABSTRACT: Silica gel column chromatography, gas chromatography-olfactometry (GC-O) combined with GC-mass
spectrometry (GC-MS) and recombination-omission tests led to the identification of the key odor compounds in the
burnt smell of upper tobacco leaves. The results showed that a total of 16 phenolic compounds were identified
from the extracts of the burnt smell components (EBSC best power station), 10 of which were identified as important odorants in
EBSC with odor activity values (OAVs) greater than 1. In addition, the recombination tests showed that there was no
significant difference in the five odor attributes between the original EBSC and the recombinant model, proving the
successful simulation of the typical odors of EBSC. The omission tests demonstrated that the key odor compounds in
EBSC (p < 0.05) were o-cresol, p-cresol, 2,6-dimethylphenol, 2-ethylphenol, 2,4-dimethylphenol, 4-ethylphenol, 3,5-
dimethylphenol, and 2,4,6-trimethylphenol. Further studies on the characterization of key odor compounds in EBSC will
be helpful to the directional elimination of the burnt smell from upper tobacco leaves.
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| a |
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450001 China |
| b |
Inner Mongolia Kunming Cigarette Co., Ltd., Hohhot, Inner Mongolia 010020 China |
* Corresponding author, E-mail: li867057788@163.com, liangmiaozzu@163.com
Received 6 Apr 2022, Accepted 6 Dec 2022
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