Research articles
ScienceAsia 49 (2023): 248-255 |doi:
10.2306/scienceasia1513-1874.2023.009
In vitro antimicrobial examination and efficacy of Eryngium
foetidum L. extract for skin ointment in animal infectious
dermatitis treatment
Korawuth Punareewattanaa, Glenn N. Borlaceb, Supawadee Seubsasanac, Eakachai Thongkhama,Jareerat Aiemsaarda,*
ABSTRACT: The development of new antimicrobial formulations for the treatment of animal infectious dermatitis
is important as current antimicrobial treatments can have undesirable effects. The purpose of this study was
to examine the in vitro antimicrobial activity of an Eryngium foetidum L. ethanolic extract against a bacterium,
Staphylococcus pseudintermedius; a yeast, Malassezia pachydermatis; and three dermatophytes, Microsporum canis,
Microsporum gypseum, and Trichophyton mentagrophytes; all of which are the most common animal skin pathogens
causing dermatitis. A viscous semi-solid crude extract was obtained at a yield of 30.6% dry weight. Quantitative
analysis of the chemical constituents of the extract by spectrophotometry revealed 3.62 mg GAE/g extract of total
phenolic, 1.77 mg CE/g extract of total flavonoid, and 10.11 mg/g extract of total carotenoid contents. HPLC-DAD
analysis of the carotenoid content showed that ?-carotene and lutein were present at 5.17 and 4.43 mg/g extract,
respectively. Antimicrobial activity by broth microdilution test found that the E. foetidum extract had MIC, MBC and
MFC values in the range of 6.25 to 25.0 mg/ml; and the time-kill test indicated that this effect was both dose and
time dependent. Ointment formulations containing the extract at concentrations of 5, 7.5 and 10% w/w had high
microbicidal activity against the tested pathogens and retained their activity and physical properties after repeated
freeze-thaw cycles. The results of this study show the potential of E. foetidum extract to be developed as a novel
antimicrobial formulation for treatment of infectious dermatitis in pets.
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| a |
Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002 Thailand |
| b |
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 Thailand |
| c |
Faculty of Pharmacy, Thammasat University, Pathum Thani 12120 Thailand |
* Corresponding author, E-mail: jaraim@kku.ac.th
Received 27 Feb 2022, Accepted 16 Oct 2022
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