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Expression analysis of silkworm genes related to juvenile hormone during the BmCPV infection

Runzhen Chen^a,b,?, Ling Wang^a,b,?, Bin Li^a,b, Xijie Guoa,b, Kun Gao^a,b,*

 
ABSTRACT:     Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the most important pathogens of silkworm diseases, which infects the midgut epithelial cells of silkworm larvae. Previous results on isobaric tags for relative and absolute quantitation and digital gene expression showed that certain genes and proteins related to the synthesis, metabolism, and signaling pathway of juvenile hormone (JH) were expressed differentially during BmCPV infection. To investigate the relationship between BmCPV infection and JH regulatory mechanisms, quantitative real-time PCR was used to further detect the relative expression levels of some genes. Results showed that the genes involved in JH synthesis and signaling pathway were up-regulated in the BmCPV-infected midgut of silkworm, whereas those involved in JH metabolism were mainly down-regulated in the midgut after BmCPV infection. JH binding protein (JHBP) is the receptor protein that binds to JH, and its up-regulation of 40.7 times in BmCPV-infected silkworm at 120 hpi was closely related to the high concentration of JH. Thus, BmCPV infection could increase JH concentration by inducing synthesis or reducing the degradation of JH. High concentration of JH in the late stage of BmCPV-infected silkworm prolonged the larval stage of silkworm by participating in signal transduction pathway, providing a condition conducive to virus replication. The results would provide not only insights into the response mechanism of hormone regulation in silkworm during virus infection but also new ideas regarding the biological activities of candidate genes and defense mechanism in silkworm against BmCPV infection.

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* Corresponding author, E-mail: gkunjn2002@126.com

Received 16 Aug 2021, Accepted 2 Mar 2022