Research articles
ScienceAsia 48 (2022):ID 270-277 |doi:
10.2306/scienceasia1513-1874.2022.047
Analysis of novel prognostic markers of oral squamous cell
carcinoma at the transcriptome-wide level
Li Kunshana, Liu Yuanhangb, Sun Jiemingc, Qiu Junpingc, Wang Wenjinga, Qiu Yonglea,*
ABSTRACT: Patients with oral squamous cell carcinoma (OSCC) have a poor prognosis. The molecular mechanism
of OSCC progression remains unclear. The aim of this study was to explore specific prognostic biomarkers of OSCC
at the transcriptome-wide level. A total of 158 differentially expressed long non-coding RNAs (lncRNAs), including
85 cis-acting and 10 trans-acting differentially expressed lncRNAs, were screened in OSCC tissues compared with
adjacent normal tissues. Both cis- and trans-target genes of differentially expressed lncRNAs were enriched in pathways
associated with cancer progression. Additionally, 25 differentially expressed mRNAs were screened. Overlapping
analysis of differential protein-coding genes with the cis- and trans-target genes of differentially expressed lncRNAs
indicated that KIF19 was the only trans-acting gene of differentially expressed lncRNA ?ENST00000626052? (named
ENSTa). ENSTa was down-regulated in OSCC tissues and cells, and its low expression corresponded to a poor
prognosis in OSCC. KIF19 was highly expressed in OSCC tissues and cells. Patients with high expression of KIF19
had a poor prognosis. Cellular experiments showed that KIF19 expression and cell proliferation were inhibited in
cells overexpressing ENSTa. However, KIF19 overexpression counteracted the effect of ENSTa overexpression on cell
proliferation. In conclusion, ENSTa regulated the trans-acting KIF19 gene in the inhibition of the progression of OSCC,
thus they could be suggested as novel biomarkers for OSCC.
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a |
Department of Stomatology, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei 050000 China |
b |
Department of Stomatology, Second Hospital of Shijiazhuang, Shijiazhuang, Hebei 050000 China
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c |
Department of Stomatology, Xianghe County People?s Hospital, Langfang, Hebei 065400 China |
* Corresponding author, E-mail: dentist2020@163.com
Received 2 Aug 2021, Accepted 26 Nov 2021
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