Research articles
ScienceAsia 46 (2020):ID 30-36 |doi:
10.2306/scienceasia1513-1874.2020.007
A novel silkworm pupae carboxymethyl chitosan
inhibits mouse L929 fibroblast proliferation
Lin Zhu, Zuo-Qing Fan, Xin-Qin Shi, Na Wang, Ying-Ying Bo, Hong-En Guo*
ABSTRACT: Postoperative intestinal adhesions are common and serious complications after surgery that can cause pain
and potential mortality. Our previous study confirmed that silkworm pupae carboxymethyl chitosan (SP-carboxymethyl
chitosan) reduced postoperative adhesion in vivo. Here, we elucidated the inhibitory effects of SP-carboxymethyl
chitosan on mouse L929 fibroblasts. Cells were exposed to SP-carboxymethyl chitosan for 72 h, then the inhibitory
effects were assessed via transforming growth factor-β1 (TGF-β1)/Smads, plasminogen activator inhibitor 1 (PAI-1),
and tissue-type plasminogen activator (t-PA) signaling. The results showed that SP-carboxymethyl chitosan suppressed
cell hyperplasia and significantly attenuated the gene and protein expressions of the TGF-β1/Smads signaling pathways.
We also confirmed that t-PA/PAI-1 greatly increased for all SP-carboxymethyl chitosan-treated groups compared to
the control. These findings suggest that SP-carboxymethyl chitosan may affect L929 cell proliferation through the
TGF-β1/Smads signaling pathway to prevent adhesion after an operation.
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Shandong Institute of Sericulture, Yantai 264001 China |
* Corresponding author, E-mail: guohongen@shandong.cn
Received 9 Jul 2019, Accepted 4 Jan 2020
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