Research articles
ScienceAsia (): 509-514 |doi:
10.2306/scienceasia1513-1874...509
Analysis of nutritional composition, antioxidant
activity and callus induction of Oryza sativa cultivars
Khumthan and Norprae
Natthiya Chaichana
ABSTRACT: The purpose of this study was to investigate the nutritional composition, antioxidant activity and callus
induction of Oryza sativa cultivars Khumthan and Norprae, which are the local rice cultivars in Chiang Saen district,
Chiang Rai province, Thailand. Nutritional information was examined by AOAC method. It was found that fat, proteins,
carbohydrates, crude fibre and ash composition of both rice cultivars were varied in the range of 2.56–2.91, 6.53–9.09,
71.87–76.36, 1.60–2.03, and 1.14–2.10%, respectively. Furthermore, twelve kinds of minerals including N, P, K, Ca,
Mg, Mn, S, B, Na, Fe, Zn, and Cu were also detected in both cultivars. For antioxidant analysis, O. sativa cultivar
Norprae had antioxidant activity of 19.39 µmol TE/g, while cultivar Khumthan had the lower value of antioxidant
activity (2.4 µmol TE/g). Callus induction of both cultivars was examined. The seeds were surface sterilized with
15% Clorox for 10 min. The sterilized seed embryos were transferred to MS (Murashige and Skoog, 1962) medium
supplemented with 0, 0.5, 1.0, 2.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic acid) or kinetin. The result revealed that MS
medium supplemented with 0.5 mg/l 2,4-D provided the highest callus induction of cultivars Khumthan and Norprae
with 1.344 and 1.385 g fresh weight, respectively.
Download PDF
214 Downloads 1412 Views
a |
Science Program, Faculty of Education, Chiang Rai Rajabhat University, Chiang Rai 57100 Thailand |
* Corresponding author, E-mail: natthiya.cha@crru.ac.th
Received 2 Apr 2019, Accepted 10 Oct 2019
|