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Short Report
ScienceAsia 34 (2008): 107-113|doi: 10.2306/scienceasia1513-1874.2008.34.107
Kinetic Spectrophotometic Determination of Acetylcysteine and Carbocisteine in Bulk Powder and in Drug Formulations
Elham Anwer Tahaa*, Nagiba Yehya Hassanb, Fahima Abdel Aala and Laila El-Sayed Abdel Fattahb
ABSTRACT: A simple, sensitive and validated kinetic spectrophotometric method for the determination of certain mucolytic drugs namely acetylcysteine (Ac) and carbocisteine (Cc) is described. This method is based on the kinetic investigation of the reaction between the two cited drugs and 4-chloro-7-nitrobenzo-2-oxa 1, 3 -diazole (NBD-Cl) in an alkaline medium. Spectrophotometric measurements were achieved by recording the absorbance at 424 nm at ambient temperature (250C ±5) for a fixed time of 30 minutes for Ac, and at 468 nm for a fixed time of 15 minutes at 70 0C for Cc. All variables affecting the development of the reaction were investigated and optimized. The method was found to be linear over the range of 2-22 μg ml-1 for Ac, and 5- 35 μg ml-1 for Cc with mean percentage recoveries of 99.59 ± 0 .41 and 99.50 ± 0.65, respectively. Molar absorpitivity and sensitivity index were determined and found to be (1.094x103 and 1.49x10-2, respectively, for Ac, and 2.547x103 and 3.05x10-2, respectively for Cc. The validity of the method was assessed according to USP guidelines. The proposed method was successfully applied for the determination of the two cited drugs in bulk powder, in pharmaceutical formulations as well as in the presence of their related substances. The results obtained were found to agree statistically with those obtained by official and reported methods. In this study the determination of the two drugs by the fixed time method proved to be more applicable than the fixed absorbance and rate constant methods.
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a National Organization for Drug Control and Research (NODCAR), 6Abou Hazem Pyramids, P.O.Box 29 Giza, Egypt.
b Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Aini street, Cairo11562, Egypt.
* Corresponding author, E-mail: dr_elhamtaha@hotmail.com
Received 2 Feb 2007, Accepted 3 Sep 2007
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