Establishment of in vitro Culture of Musa AA Group ‘Kluai Sa’ and Musa AA Group ‘Kluai Leb Mue Nang’ and the Analysis of Ploidy Stability

Amornwat Srangsam and Kamnoon Kanchanapoom*

ABSTRACT: The use of shoot tip culture for ‘Kluai Sa’ (Musa AA group ‘Kluai Sa’) and ‘Kluai Leb Mue Nang’ (Musa AA group ‘Kluai Leb Mue Nang’) banana plant propagation is described. Excised shoot apices were cultured on MS (Murashige and Skoog, 1962) medium supplemented with 3 mg/l BA (6-Benzyladenine) and then transferred to MS medium containing 100 mg/l casein hydrolysate, 15% CW (Coconut water) and various concentrations of BA. The maximum shoot numbers were obtained on the medium containing 5 mg/ l BA with 4.9 shoots in ‘Kluai Sa’ and 5.5 shoots in ‘Kluai Leb Mue Nang’. Root and callus formation were achieved when shoot buds were planted on MS media containing various concentrations of NAA (1- Naphthaleneacetic acid) in combination with 5 mg/l BA. Adventitious shoots were also regenerated from calli that were cultured on MS medium containing 5 mg/l BA and 0.1 mg/l NAA. All regenerated shoots rooted well on MS medium either supplemented with or without 0.2% activated charcoal. More than 95% of the surviving plantlets were acclimatized and successfully transferred into soil. Cytological and flow cytometric analyses of the mother plants and micropropagated plants derived from 2 years-old cultures of both cultivars showed no differences in ploidy level, they were all diploid (2n = 2x = 22) with a 2C peak.

Download PDF

Department of Biology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.

* Corresponding author, E-mail: kamnoon.k@psu.ac.th

Received 13 Nov 2006, Accepted 1 Aug 2007