ScienceAsia 33 (2007): 347-352 |doi: 10.2306/scienceasia1513-1874.2007.33.347

Detection of Brown Planthopper Resistance Genes from Different Rice Mapping Populations in the Same Genomic Location

Jirapong Jairin,^a* Sanguan Teangdeerith,^a Phikul Leelagud,^a Kittiphong Phengrat,^a Apichart Vanavichit^b and Theerayut Toojinda^b

 
ABSTRACT:     Three rice mapping populations of 208 BC₁F₂, 333 BC₃F₂ and 335 F2 lines derived from crosses of PTB33 × RD6, Rathu Heenati × KDML105 and IR71033-121-15 × KDML105, respectively, were used to detect brown planthopper (BPH) resistance genes. The modified mass tiller screening (MMTS) method was applied to evaluate the BPH resistance of all mapping population lines at the tillering stage. The BPH resistance genes detected from the BC₁F₂, BC₃F₂ and F2 populations were mapped in the same genomic region on the short arm of chromosome 6. The tightly linked markers RM589 and RM586 could explain 59.8%, 28.2% and 57.4 % of the phenotypic variance of the BPH resistance from the BC₁F₂, F2 and BC₃F₂, respectively. The tightly linked SSR markers identified from this study should be useful in marker-assisted breeding to produce BPH resistant cultivars.

KEYWORDS: BPH resistance gene, modified mass tiller screening (MMTS), Nilaparvata lugens, Oryza minuta, SSR marker.

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^a Ubon Ratchathani Rice Research Center, P.O. Box 65, Muang Distric, Ubon Ratchathani 34000, Thailand.
^b Rice Gene Discovery Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

* Corresponding author, E-mail: jirapong@ricethailand.go.th

Received 13 Sep 2006, Accepted 12 Apr 2007