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Research Article
ScienceAsia 31 (2005): 341-348 |doi: 10.2306/scienceasia1513-1874.2005.31.341
Optimized and Validated Spectrophotometric Methods
for the Determination of Verapamil Hydrochloride in
Drug Formulations
Nafisur Rahman*, Nadeem Ahmad khan and Syed Najmul Hejaz Azmi
ABSTRACT: Two spectrophotometric methods have been described for the assay of verapamil hydrochloride in
drug formulations. Method A is based on the oxidation of the drug with potassium metaperiodate in sulfuric
acid medium to give colored product, which absorbs maximally at 425 nm. Method B involves the formation
of colored chloroform extractable ion-pair complex of the drug with tropaeolin 000 No.1 at pH 4.0 showing
absorption peak at 400 nm. Under the optimized experimental conditions, Beer’s law is obeyed in the
concentration ranges of 12.5-187.5 and 2.0-30.0 ?g mL-1 for methods A and B, respectively. Results of
analyses were validated statistically and through recovery studies. Statistical comparison of the results with
the reference method shows excellent agreement and indicates no significant difference in accuracy and
precision. Both methods have been successfully applied to the determination of verapamil hydrochloride in
drug formulations.
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Department of Chemistry, Aligarh Muslim University, Aligarh-202002 (U.P.) India.
* Corresponding author, E-mail: cht17nr@yahoo.co.in
Received 5 Apr 2005,
Accepted 16 Jun 2005
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