Gamma Radiation Induced Mutations in Mungbean

Chontira Sangsiri,^a Worawit Sorajjapinun,^b and Peerasak Srinives^c*

ABSTRACT: Seeds of mungbean varieties KPS 2, VC 6468-11-1B, their F₁ and F₂ were treated with gamma rays (Cs-137 source) at the dose of 500 Gy. The M1 seeds were sown in the field with the controls (non-irradiated seeds) and bulk-harvested. The M₂ seeds were sown to observe their characters and number of mutants in each population. Among over 430,000 plants observed, irradiated F₁ population gave the highest frequency of mutants at 0.168%, followed by F₂, VC 6468-11-1B, and KPS 2 at 0.165%, 0.152%, and 0.142%, respectively. Mutant characters were grouped as chlorophyll, leaf, flower, and pod mutants. Chlorophyll mutations included albino, coppery leaf, light-green leaf, variegated leaf, waxy leaf, white streak leaf, and xantha leaf. Leaf mutations were lanceolate leaf, narrow-rugose leaf, multiple leaflet, round-cuneate leaf, unifoliate leaf, and wrinkled leaf. The flower mutant was cock’s comb raceme while the pod mutant was a lobed one. All mutants were purified for genetic study and possible uses of the traits.

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a Center for Agricultural Biotechnology Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
b Asian Regional Center AVRDC - the World Vegetable Center, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
c Department of Agronomy Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

* Corresponding author, E-mail: agrpss@yahoo.com

Received 6 Jan 2005, Accepted 25 May 2005