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Research Article
ScienceAsia 30 (2004): 335-340 |doi: 10.2306/scienceasia1513-1874.2004.30.335
Distribution and Substrate Specificity of D-Amino Acid
and D-Aspartate Oxidases in Marine Invertebrates
Mohammed Golam Sarower,a,b* Takashi Matsuia and Hiroki Abea
ABSTRACT: The distribution of D-amino acid oxidase (DAO, EC 1.4.3.3) and D-aspartate oxidase (DDO, EC
1.4.3.1) activities was examined on several tissues of various marine invertebrate species. Only DAO activity
was detected in kidney and midgut gland from most of the mollusks with some exceptions. Hepatopancreas
or liver from kuruma prawn, crayfish, squid, and octopus and antenal gland from crayfish showed significant
amount of activity of both enzymes. Squid demonstrated a higher activity of DDO than that of DAO. DProline
was the best substrate for DAO in common flying squid liver followed by D-alanine, D-histidine, Dtyrosine,
and D-phenylalanine. On the contrary, meso-2,3-diaminosuccinate was the best substrate for DDO
followed by N-acetyl-DL-aspartate, D-glutamate, D-amino adipate, and D-homocysteic acid. These enzymes,
at least in kidney and midgut gland, are believed to metabolize exogenous and endogenous free D-alanine,
that is abundant in aquatic invertebrates such as crustaceans and bivalve mollusks.
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a Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
b Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna-9208, Bangladesh.
* Corresponding author, E-mail: sarower@gawab.com
Received 13 May 2004,
Accepted 27 Sep 2004
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