 Back
Research Article
ScienceAsia 30 (2004): 231-237 |doi: 10.2306/scienceasia1513-1874.2004.30.231
Characterization and Protection in Mice of Outer
Membrane Proteins from Pasteurella multocida A:1
Incorporated in Lipid Vaccine Delivery Systems
Atthachai Homhuan,a* Pornpen Pathanasophon,b Daan JA Crommelin,c Wim Jiskoot,c Gideon
FA Kerstend and Sompol Prakongpana
ABSTRACT: Fowl cholera is an infectious disease affecting poultry and is caused by Pasteurella multocida. To
develop a subunit vaccine, outer membrane proteins (OMPs) from P. multocida serotype A:1 strain NIAH
DU1551/97 were extracted and characterized using SDS-PAGE. The OMPs were solubilized in detergent or
incorporated in lipid-based antigen delivery systems, namely virosomes made from Newcastle disease (ND)
virus and immunostimulating complexes (ISCOMs). The formulations were characterized by several
physicochemical methods. To evaluate the formulations as possible vaccine, their potency was tested in
mice. Eleven proteins that range in size from 30 to 80 kDa were detected in the OMP fraction. The most
prominent protein bands were 30, 33, and 45 kDa. Virosomes and ISCOMs showed an average diameter of
180 and 30 nm, respectively. OMPs incorporated in the virosomal membrane inhibited the capability of
virosomes to agglutinate chicken red blood cells. Animals were challenged after two immunizations. All
vaccines fully protected mice against a low dose challenge. In the case of a high dose challenge, micellar
OMPs provided 80% protection, whereas OMPs incorporated in virosomes or ISCOMs gave 100% protection,
which is comparable to that of inactivated whole cell vaccines. In conclusion, antigen loaded virosomes and
ISCOMs are potential pasteurella subunit vaccines.
Download PDF
a Department of Pharmacy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
b Bacteriology Section, National Institute of Animal Health, Bangkok 10900, Thailand.
c Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Utrecht Institute for
Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands.
d Research and Development Unit, Netherlands Vaccine Institute, P.O. Box 457, 3750 AL Bilthoven, The
Netherlands.
* Corresponding author, E-mail: g4138458@yahoo.com
Received 20 Jan 2004,
Accepted 21 May 2004
|
