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Research Article
ScienceAsia 30 (2004): 223-230 |doi: 10.2306/scienceasia1513-1874.2004.30.223
Characteristics and Distribution of Actinophages
Isolated from Thai Soil Samples
Onanong Pringsulaka,a Surina Chavanich,b Katsumi Doic and Seiya Ogatac
ABSTRACT: Indigenous streptomycetes isolated from soil samples collected from several parts of Thailand were
used as hosts for isolation of phages. Twenty-four phages were obtained by an enrichment procedure. The
phages were plaque purified and morphologically examined under a transmission electron microscope. It
was observed that all phages had hexagonal heads and long non-contractile tails. Thus, they belonged to
group B of Bradley’s classification and type B1 in the Family Siphoviridae. The host range of the phages was
determined against 163 streptomycete strains and was found to vary from narrow to broad. Ten phages were
characterized based on their morphology, physiology, genome, proteins, antigenic relationship and biology.
Phages No.1 and No.15, which had broad host ranges, showed dissimilar patterns in the one step growth
experiment. The majority of phages gave maximum replication at neutral pH although phage No.15
preferred basic conditions. Phages No.30 and No.27 were closely related serologically. The number and size
of fragments obtained after digestion with restriction enzymes showed that all phages were unique. The
results showed that they were distinguished from previously reported phages.
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a Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
b Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
c Microbial Genetic Division, Institute of Genetic Resources, Kyushu University, 812-8581, Japan.
* Corresponding author, E-mail: opringsulaka@hotmail.com
Received 3 Nov 2003,
Accepted 18 May 2004
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