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Research Article
ScienceAsia 30 (2004): 123-126|doi: 10.2306/scienceasia1513-1874.2004.30.123
Coastal Soil Actinomycetes: Thermotolerant Strains
Producing N-Acylamino Acid Racemase
Rattanaporn Srivibool,a,* Kimiko Kurakami,b Morakot Sukchotiratanac and Shinji Tokuyamab
ABSTRACT: Sixty four soil samples collected from the coastal ecosystem of Chonburi, Rayong and Trat
Provinces were isolated for thermotolerant actinomycetes. Among 567 isolates of thermotolerant strains,
12 appeared to be N-acylamino acid racemase producers at various quantities. From the 16S DNA gene
sequence and morphological characteristics strain Sal35-16, the highest enzyme producer, was identified as
Streptomyces tendae. By measuring the end product, this S. tendae could produce L-methionine at 0.68 mM.
Another high enzyme producer, strain Sal31-15, identified as Streptomyces maritimus, produced 0.21 mM Lmethionine
and 5.79 mM D-methionine. Both strains had spiral spore in chains with warty spores. Strain
Sal35-16 and strain Sal31-15 grew well between 30-50 oC and 30-45 oC, respectively. Both strains were
capable of producing N-acylamino acid racemase, not previously known from these species of Streptomyces.
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a Institute of Marine Science, Burapha University, Chonburi 20131, Thailand.
b Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka,
Japan.
c Department of Biology,Faculty of Science, Chiang Mai University, Chiang Mai 50202, Thailand.
* Corresponding author, E-mail: rattanap@bucc.buu.ac.th
Received 31 Jul 2003,
Accepted 25 Mar 2004
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