 Back
Research Article
ScienceAsia 27 (2001) : 075-081 |doi: 10.2306/scienceasia1513-1874.2001.27.075
Application of Cell Culture From Hybrid Catfish ( Clarias gariepinus X Clarias macrocephalus) in Screening Toxicity of Chemicals
Pornsawan Visoottivisetha,* and Nantawan Chanwannab
ABSTRACT: Three types of primary cell cultures were established from organs of hybrid catfish (Clarias gariepinus x Clarias macrocephalus) namely, caudal trunk, gill and liver. Cell cultures from caudal trunk and gill were composed of mixed cells of fibroblasts and epitheloids, while cell cultures from the liver were composed of fibroblastic cells only. All cultures grew well in either Eagle’s essential medium (MEM) supplemented with 15-20% fetal calf serum (FCS) or Leibovitz’s l-15 medium supplemented with 5- 20% FCS. The optimum growth temperature was 27oC in an atmosphere either with or without 5% carbon dioxide. The modal chromosome number as determined from the 19th passage of caudal trunk cell culture was 56. The cell cultures could be stored at 4oC for at least 2 months. Experiments were then performed to investigate the feasibility of the established catfish cell culture for screenin g toxicityof organotin pesticides (triphenyltin hydroxide, TPTH). The results showed that the culture was highlysuitable for the purpose. The effect of TPTH on cell culture was recognized at the concetration of 3 mg l-1 when cell detachment was used as the criterion of the test. Established cell culture from ybrid catfish has great potential as a powerful tool in tropical aquatic toxicology as a method of screening chemicals prior to in vivo testing.
Download PDF
a Biology Department, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
b Chulabhorn Research Institute, Bangkhen, Bangkok 10200, Thailand.
* Corresponding author, E-mail: scpvi@mahidol.ac.th
Received 2 Feb 2000, Accepted 7 Feb 2001
|
