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Research Article
ScienceAsia 26 (2000) : 207-211 |doi: 10.2306/scienceasia1513-1874.2000.26.207
Mechanisms of Adaptation to Increasing Salinity
of Mulberry: Proline Content and Ascorbate
Peroxidase Activity in Leaves of Multiple Shoots
Poontariga Harinasut a,*, Supaporn Srisunaka, Siriporn Pitukchaisopola and Rungsi Charoensatapornb
ABSTRACT: Multiple shoots of mulberry (Morus alba cv Khonpai), a NaCl-tolerant cultivar, were grown
on MS media without or with NaCl. On medium with increasing NaCl concentration up to 150 mM, the
shoots exhibited slightly reduction in growth. On the other hand, when the concentration of NaCl
increased to 200 mM, the growth was completely inhibited and the necrosis of leaves tissue was observed.
The relative water content was significantly decreased in leaves treated with NaCl concentration of 150
and 200 mM compared to that in leaves without NaCl treatment. The total chlorophyll content was
significantly lower in leaves of salt treated multiple shoots than in leaves of control multiple shoots.
Comparing shoots grown on media without to with 150 mM NaCl, the proline content in leaves was
increased to approximately 11-fold and the ascorbate peroxidase activity in leaves was increased to
approximately 91%.
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a Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
b Plant Pathology and Microbiology Division, Department of Agriculture, Ministry of Agriculture and Cooperatives, Bangkok 10900, Thailand.
* Corresponding author, E-mail: fscipth@nontri.ku.ac.th
Received 16 Mar 2000, Accepted 30 Nov 2000
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