ScienceAsia 26 (2000) : 201-205 |doi: 10.2306/scienceasia1513-1874.2000.26.201

A Protocol Towards Micropropagation of the Piscicidal Plant, Maesa ramentacea A. DC.

Kamnoon Kanchanapoom^a,*and Kantamaht Boonvanno^b

ABSTRACT: A tissue culture system was established from shoot tips of the piscicidal plant, Maesa ramentacea A. DC. Shoot proliferation was achieved with Murashige and Skoog (MS) medium supplemented with either BA, BA-Kinetin or BA-NAA combinations. Stocking of shoot cultures was obtained by repeating subculture in the same medium at 30-day intervals wherein the rate of multiplication was maintained. For rooting, the individual shoot was implanted on root induction medium consisted of MS medium supplemented with 2 mg/l NAA. Within two weeks of incubation 100% rooting was evidenced. Complete plantlets were acclimatized and successfully transplanted to soil.

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a Department of Biology, Faculty of Science, Prince of Songkla University Hat Yai, Songkhla 90112, Thailand.
b Natural History Museum, Faculty of Science, Prince of Songkla University Hat Yai, Songkhla 90112, Thailand.


* Corresponding author, E-mail: kkamnoon@ratree.psu.ac.th

Received 6 Sep 1999, Accepted 30 Nov 2000