ScienceAsia 22 (1996): 217-226 |doi: 10.2306/scienceasia1513-1874.1996.22.217

UTILIZATION OF TISSUE CULTURE TECHNIQUE FOR PROPAGATION OF MELIA AZEDARACH

SARINEE CHAICHAROEN^a, SURlYA JANSAENGSRI^b, THATCHAI UMPRAI^c AND MALEEYA KRUATRACHUE^a

ABSTRACT: The modified LS basal medium used in this study was composed of macronutrients, micronutrients from LS medium, vitamins of B5 proposed by Gamborg, and 10% coconut water. The explants of Melia azedarach used in this experiment were young leaf, petiole, internode, terminal bud and axillary bud from young plants. The optimum concentration of BA for multiple shoot induCtion from axillary and terminal bud was 1 mg/l. Callus cultures could be readily initiated and proliferated from petiole and internode in the basal medium supplemented with 1 mg/l NAA plus 1 mg/l BA. The derived callus cultured on basal medium supplemented with 1 mg/l BA regenerated shoots which had a vigorous growth in basal medium with 0.25 mg/l BA. The individual shootlus regenerated vigorous roots within 7-10 days afier immersion of the basal cut end into the solution of 1 mg/l IAA and 1 mg/l IBA for 1 day, then transferred to basal medium; 85% of the treated shoots rooted. One-month-old plantlets were then transplanted into pots filled with sterilized vermiculite under high humidity conditions. The survival percentage was about 70%. Complete plants were grown in the field.

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a Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
b Roi-ed Sericultural Experiment Station, Roi-ed, Thailand.
C Ministry of Forestry, Bangkok, Thailand.

Received April 3, 1995