ScienceAsia 22 (1996): 031-041 |doi: 10.2306/scienceasia1513-1874.1996.22.031

A NEWLY ESTABLISHED CERVICAL CARCINOMA CELL LINE FROM A THAI PATIENT

YINGPAN ANUSRI^a, BOONRAK PHUNCHAISRI^d, VIPAVADEE CHAISUKSUNT^a, NIRUTH LERTPRASERTSUKE^b,VICHARN LORVIDHAYA^c, THIRAPHAT VILAITHONG^d, AND SUPARP NA CHIANG MAI^e

ABSTRACT: A cell named IST3 was established from a squamous carcinoma of the uterine cervix from a Thai patient for use as a biological model for studying the effects of neutron radiation on the cell with and without boron compound. This project is part of Thailand's BNCT project. The doubling time of this cell line is 24 hours at passage 5. The cells have been grown well without interruption (January - July 1994) for 30 passages. Chromosome analysis at passage 14 showed numerical abnormality, 80% of the cells are polyploids, in a range of 71-98 with a modal number of 92. More than 40% of metaphases had somewhat similar two marker chromosomes which suspected to be derived by reciprocal translocation. Phase contrast microscopy revealed polygonal and pleomorphic in size and shape. Under the scanning electron microscope, the cells showed polygonal shape. with numerous very long processes at the cell border. Interconnection between the tip of the processes of adjacent cells was observed. Transmission electron microscopy revealed desmosomes and tonofilaments of the cell thereby confirming its epithelial nature. Immunocytochemical staining of cytokeratin showed positive reaction of the epithelial cell type. Xenograft in nude mice is studied.

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a Department of Anatomy, b Department of Pathology and c Department of Radiation, Faculty of Medicine, Chiang Mai University,Chiang Mai 50200, Thailand.
d Institute for Science and Technology Research and Development, Chiang Mai University, Chiang Mai 50200, Thailand.
e Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.

Received Aug 3, 1995