 BackResearch Article ScienceAsia 21 (1995): 175-182 |doi: 10.2306/scienceasia1513-1874.1995.21.175
CONDUCTIVITY MEASUREMENT OF THE INHIBITORY EFFECT OF HEAVY METAL IONS ON IMMOBILIZED UREASE
PANOTE THAVARUNGKUL1, PROESPICHAYA KANATHARANA2, NATTAWUT NIPANUN2, JITSANU PETCHARAT2 AND PUNNEE ASAWATRERATANAKUL3ABSTRACT: The inhibitory effect of heavy metals on the enzymic reaction of urease was determined by comparing the conductivity peaks of sample solutions resulting from hydrolysis of urea into charged products, before and after the introduction of inhibitors. A linear relationship between percentage of inhibition and concentration of heavy metal ions was observed with concentrations in the range of 10-4-10-3 M for copper(II) and 10-6-10-5 M for mercury (II). The enzyme column can be regenerated by the passage of a solution of NaI followed immediately by EDTA. 1 Department of Physics, 2 Department of Chemistry, 3 Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla 90112, Thailand Received March 29, 1995
|
