 BackResearch Article ScienceAsia 21 (1995): 137-146 |doi: 10.2306/scienceasia1513-1874.1995.21.137
IN VITRO PLANT REGENERATION THROUGH YOUNG LEAF CULTURE IN MULBERRY (MORUS ALBA VAR. 554 X MORUS ALBA VAR. NOI)
SARINEE CHAICHAROENa AND SOMPOTI AKRAPANTHUbABSTRACT: Young leaf explants of mulberry (Morus alba var. S54 x Morus alba var. Noi), derived from aseptically grown shootst were cultured on the modified Murashige and Skoog medium supplemented with various concentrations of N6-Benzyladenine (BA) and 1-Naphthaleneacetic acid (NM). The optimum concentrations of BA and NAA for cal/us initiation and proliferation were 0.5 mg/l BA plus 1.0 mg/l NM and 1.0 mg/l BA plus 0.5 mg/l NAA respectively. The Callus was subcultured on the medium containing 1.0 mg/l BA and 0.025 mg/l NM for shoot regeneration. To obtain shoot growtht the regenerated shoots were cultured on the medium supplemented with 0.25 mg/l BA. A root formation occurred when culturing an individual shoot on half-strength basal medium in the presence of 0.1 mg/l Indole-3-butyric acid (IBA). The addition of 0.1 % activated charcoal was able to absorb browning substance during shoot growth and root formation stages. The plantlets were first transferred to sterilized vermiculite and then in the clay pots with soil. The complete plants 1-1.5 feet high have been grown in a trial field. a Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Received March 1, 1995
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