 BackShort Report ScienceAsia 19 (1993): 097-102 |doi: 10.2306/scienceasia1513-1874.1993.19.097
TRANSCRIPTION OF "SLOW VIRUS" DEMENTIA PRION PROTEIN GENE IN LYMPHOCYTES OF HUMAN BLOOD
BANTHIT CHETSAWANG,1 HELEN RIOL,2 GEORGES LEVESQUE2 AND M.R. VEN MURTHY2ABSTRACT: We have analysed illegitimate transcription of brain specific genes in normal human blood Iymphocytes. Total RNA was extracted from Iymphocytes and after a first step of reverse transcription, the prion cDNA sequence was amplified by polymerase chain reaction (PCR) in the presence of specific primers. Amplification of a fragment of the prion gene sequence was observed, indicating that the corresponding gene was transcribed in blood Iymphocytes. 1 Neurobehavioural Biology Center, Institute of Science and Technology for Development, Mahidol University, Salaya, Nakornpathom 73170, Thailand. 2 Department of Biochemistry, Faculty of Medicine, Laval University, Quebec G1K 7P4, Canada. Received May 3, 1993
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