 BackResearch Article ScienceAsia 19 (1993): 047-055 |doi: 10.2306/scienceasia1513-1874.1993.19.047
SIMPLE NON-RADIOISOTOPIC DNA FINGERPRINTING WITH DIGOXIGENIN LABELLED M13 PHAGE USING CHROMOGENIC AND CHEMILUMINESCENT DETECTION SYSTEMS
VICHAI BOONSAENG,1 ANCHALEE TASSANAKAJON,2 CHAINARONG WONGTEERASUPAYA,2 PORNPAN PUMICHOTI1 AND SAKOL PANYIM1ABSTRACT: Improved conditions for non-radioisotopic DNA fingerprinting analysis using digoxigenin M 13 probe were developed. Highly resolved banding patterns were obtained with specific-primed
labelling of M13 DNA and Photogene membrane as a blotting medium under low stringency of
hybridization conditions and post-hybridization washes. DNA fingerprinting of one family with 4 children and a pair of identical twins were performed and the signals were detected by both colourimetric and chemiluminescent detection systems. Readable pattern was generated with as little as 0.5 1 Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand. 2. Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand. Received February 5, 1993
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g of human genomic DNA This non-radioisotopic DNA fingerprinting was simple, reliable and should be applicable. as an alternative to the isotopic method.
