 BackResearch Article ScienceAsia 10 (1984): 147-178 |doi: 10.2306/scienceasia1513-1874.1984.10.147
SCREENING FOR AND ISOLATION OF UGNOCELLULOLYTIC FUNGI FROM THAILAND FOR ANIMAL FEED PRODUCTION
V. MEEVOOTISOM,a T.W. FLEGEL,a T. GLINSUKON,a N. SOBHONb and S. KIATPAPANaABSTRACT: With the ultimate objective of upgrading ruminant feed by solid fermentation, a collection of approximately 200 filamentous fungi were selected from various sources mostly in Thailand. These have been screened for cellulase activity using acid swollen (Walseth) cellulose and with cellulose azure. They have also been testedfor phenol oxidase (PO) activity using guaiacol and for growth on rice straw. Of 200 isolates 160 were able to release dye from cellulose azure and 38 of these released more dye than the reference strain Trichoderma reesei QM9414. Ten isolates decolorized the dye and this decolorization was not a pH effect. A total of 122 isolates showed PO activity and 18 of these were representatives of the genus Trichoderma. Approximately half of the isolates (99) gave some substrate weight loss when grown on rice straw screening cultures and 76% of these (75) were positive for PO activity. Ten isolates showing the greatest lignin degredation in these screening cultures were selected for toxicity tests and for retrials on 109 milled rice straw. The resulting fermentation product was analyzed for in vitro digestibility and for various components including lignin and protein. Two of the ten isolates were toxic and none of the isolates gave a product with digestibility higher than untreated rice straw, though protein content was slightly improved. a. Faculty of Science, MahidDI University, Rama VI Rd., Bangkok 10400, Thailand Received 13 December 1983
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