 BackResearch Article ScienceAsia 7 (1981): 025-032 |doi: 10.2306/scienceasia1513-1874.1981.07.025
AUTOPOLYPLOIDY IN DENDROBIUM PHALAENOPSIS
SARINEE CHAICHAROEN and KARN SAEJEWABSTRACT: This study compares the growth rates, leaf and flower morphology and pollen germination of diploid and tetraploid produced by colchicine treatment in protocorms stage of the orchid Dendrobium phalaenopsis. Protocorms of the orchid were treated in 0.05% sterile aqueous colchicine solution for 9 days. At the end of six months the seedlings were planted in the small pots and grown in the lathhouse. Chromosome counts were made from the root lips. About 50% of treated plants were tetraploid. The growth of tetraploid plants was slower than that of the diploids. The leaves of tetraploids were thicker, and they had larger guard cells, and flowers are rounder than the diploids. Meiotic division in microsporocytes of diploids and most tetraploids were normal. The germination of pollens of diploids was better than those of tetraploids. Department of Biology, Faculty of Science, Mahidol University, Bangkok 4, Thailand Received 28 November 1980
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