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Research Article


ScienceAsia 4 (1978): 036-044 |doi: 10.2306/scienceasia1513-1874.1978.04.036

 

PRODUCTION OF YEAST PROTEIN BY CANDIDA UTILIS FROM PINEAPPLE JUICE
II. BATCH CULTURE STUDY

 

SUMALEE TUNTPATCHALERN and PONG VANANUVAT

Summary: The effect of aeration during batch cultivation of Candida utilis on yeast yield and its protein content was studied in a fermenter using the pineapple juice medium supplemented with (NH4)2S04 and KH2P04 at 0.5% each. The total volume of the medium was 6 liters for each run. The agitation speed of the fermenter was kept at 1400 rpm. It was found that the yeast yield after 8 hours cultivation was highest (49.4%) at the aeration rate. of 1.0 volume of air per volume of culture medium per minute (VVM). The protein contents of the yeast are the same for aeration rates of 0.5, 1.0 and. 1.5 VVM. . The reduction of chemical oxygen demand (COD) in the supernatant liquor was also determined during cultivation of the yeast. At the end of 8 hours cultivation with 1.0 VVM aeration rate, the COD reduction was in the range 70-80 %.

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Food Technology Section, Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

Received 10 February 1978