| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 50 Number 5
Volume 50 Number 4
Volume 50 Number 3
Volume 50 Number 2
Volume 50 Number 1
Volume 49 Number 6
Earlier issues
Back

Research Article

ScienceAsia 2 (1976): 056-066 |doi: 10.2306/scienceasia1513-1874.1976.02.056

 

THE PRESENCE OF TWO IgG SUBCLASSES IN WATER-BUFFALO IMMUNOGLOBULINS

 

M.R. JISNUSON SVASTI, PRANIT PRAWATMUANG, CHAROENSRI VAJANAMARHUTUE, ANOCHA KAJADPHAI, SIRISAG WANGTHAMMANG and NAPASRI TALUPPHET

Summary: The immunoglobulins of a water-buffalo have been fractionated by ammonium sulphate precipitation and DEAE-cellulose chromatography into eight pools. These pools were analysed by polyacrylamide gel electrophoresis, analytical isoelectric focusing, hexose determination, and chemical typing. The results suggested that water-buffalo IgG consists of two subclasses, fast (IgG1) and slow (1gG2).

          In addition, the light chains were shown to be entirely of the lambda type. The C-terminal tryptic peptide. was purified and shown to contain an extra proline when compared to the equilivalent peptide from bovine light chains.

Download PDF

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand

Received 30 April 1976