BamA protein as the target of bacteriocin PA166 from Pseudomonas sp. strain 166

Yu Wang^a, Yue Ma^a, Xiaojia Fu^a, Lin Han^a, Xiaoou Zhao^b, Yanhui Chen^a,, Haiyong Guoa,^

ABSTRACT: The bacteriocin PA166 protein is secreted by Pseudomonas sp. strain 166 as previously reported by our research group. This bacteriocin is characterized by its good stability, potent antibacterial activity and low toxicity, providing valuable insights and potential opportunities for the development of novel antimicrobial agents. On this basis, we further explored the target of bacteriocin PA166. Molecular docking results showed that bacteriocin PA166 couldbindtotheextracellular membraneproteinBamA.Thereal-timequantitativereversetranscriptionPCR(RT-qPCR) result showed that after bacteriocin PA166 action on Pasteurella multocida ATCC43137 (P. multocida ATCC43137), the bamAgeneexpressionofthebacteria wassignificantly reduced. The results of the checkerboard studies showed that the activity of bacteriocin PA166 was significantly reduced by the addition of the BamA protein, thus identifying BamA as the target of the antimicrobial activity of this bacteriocin. This research not only provides an idea for the development of new antimicrobial agents, but also a useful reference for understanding their mechanism.

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^a	Jilin Normal University, SiPing, Jilin 136000 China
^b	Jilin Academy of Agricultural Sciences, ChangChun, Jilin 130033 China

* Corresponding author, E-mail: cyh@jlnu.edu.cn, guohaiyong78@jlnu.edu.cn

Received 21 Feb 2025, Accepted 16 Aug 2025

BamA protein as the target of bacteriocin PA166 from Pseudomonas sp. strain 166

Yu Wanga, Yue Maa, Xiaojia Fua, Lin Hana, Xiaoou Zhaob, Yanhui Chena,*, Haiyong Guoa,*

Yu Wang^a, Yue Ma^a, Xiaojia Fu^a, Lin Han^a, Xiaoou Zhao^b, Yanhui Chen^a,, Haiyong Guoa,^