Research articles
ScienceAsia 51 (2023): 1-8 |doi:
10.2306/scienceasia1513-1874.2023.089
Molecular detection of virulence genes and antibiotic
resistance profiles in Salmonella spp. contaminated on
food-contact surfaces in restaurant settings
Jintana Wongwigkarna, Siriwat Kucharoenphaibula, Sutthirat Sitthisaka, Rapee Thummeepaka,
Rattiya Cheewapata, Kunsuda Nimanussornkulb, Supat Khongfakc, Kannipa Tasanapaka,*
ABSTRACT: This study investigates the prevalence, virulence genes, and antimicrobial resistance profiles of Salmonella
spp. isolated from food contact surfaces and food handlers in local restaurants in northern Thailand. Salmonella, a
prominent foodborne pathogen, can thrive under diverse conditions, leading to the contamination of various food
products and causing illnesses. It is acknowledged as a prominent foodborne pathogen. A total of 650 samples were
collected, resulting in the identification of 21 Salmonella belonging to serogroups B (52.38%), D (14.30%), A, C1,
E1 (each 9.52%), and C2 (4.76%). Virulence gene analysis revealed that Salmonella group B isolates possessed the
highest number of virulence genes, with up to 12 per isolate, including commonly detected genes such as msgA, sopB,
and sifA. A significant correlation between specific virulence genes and antibiotic resistance was observed. Of the
Salmonella isolates recovered in this study, 43% were identified as multidrug-resistant (MDR). Among these MDR
strains, 78% were isolated from the hands of food handlers, while the remaining originated from kitchen surfaces.
The combined threat posed by these Salmonella strains is underscored by the presence of multiple virulence genes
and elevated levels of antibiotic resistance. Effective control measures, including stringent hygiene practices, regular
cleaning and sanitization of kitchen equipment, and the implementation of Hazard Analysis and Critical Control Points
(HACCP) systems, are essential to minimize contamination risk and prevent the spread of harmful pathogens. Ongoing
surveillance and antimicrobial resistance monitoring programs are crucial for managing the threat of MDR Salmonella
in restaurant environments.
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| a |
Department of Microbiology and Parasitology, Faculty of Medical Sciences, Naresuan University,
Phitsanulok 65000 Thailand |
| b |
Community Economics Development and Research Centre, Faculty of Economics, Chiang Mai University,
Chiang Mai 50200 Thailand |
| c |
Office of Disease Prevention and Control Region 3 Nakhon Sawan, Department of Disease Control,
Ministry of Public Health, Nakhon Sawan 60000 Thailand |
* Corresponding author, E-mail: kannipal@nu.ac.th
Received 20 Oct 2024, Accepted 0 0000
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