Research articles
ScienceAsia 52 (2026): 1-8 |doi:
10.2306/scienceasia1513-1874.2026.034
Preparation, characterization and activity verification of Xylooligosaccharides from Lablab purpureus (L.) Sweet straw
Wenming Jianga,b, Ruixue Gonga, Changyin Tanga, Rujira Sukhotuc,*
ABSTRACT: To promote the comprehensive utilization of underutilized Lablab purpureus stalk, this study innovatively
employs it as a raw material to produce xylooligosaccharides (XOS) and evaluates their antioxidant and prebiotic
activities. Hemicellulose B was extracted via alkali treatment and deproteinized using the Sevag method, as confirmed
by UV-visible spectroscopy. High-performance liquid chromatography (HPLC) analysis after hydrolysis revealed the
presence of 14 monosaccharides or derivatives, predominantly xylose and arabinose. XOS were prepared through
xylanase hydrolysis and characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy
(FT-IR), and nuclear magnetic resonance (NMR). SEM revealed a cross-linked, opaque morphology; FT-IR confirmed
the presence of ?-glycosidic bonds; and NMR indicated methyl/methoxy groups and 1?4 glycosidic linkages. The XOS
demonstrated stronger DPPH radical scavenging capacity and reducing power than hemicellulose B and effectively
promoted the growth of Lactobacillus acidophilus and Bifidobacterium animalis. This work provides a novel approach
for the high-value utilization of Lablab stalk.
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| a |
Institute of Intelligent Manufacturing and Automobile, Chongqing Chemical Industry Vocational College,
Chongqing 401228 China |
| b |
Chongqing Jiangbei District Disease Prevention Control Center, Chongqing 400020 China |
| c |
Krabi Agricultural Research and Development Center, Department of Thailand Agriculture, Krabi 81000 Thailand |
* Corresponding author, E-mail: rujira22@yahoo.com
Received 25 Jul 2024, Accepted 0 0000
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