Research articles
ScienceAsia 51 (2023): 1-10 |doi:
10.2306/scienceasia1513-1874.2023.003
Effects of 4F-phenyl pyrazole, a [6]-shogaol derivative, on
colorectal adenocarcinoma cell death
Lalida Siriananta,*, Podchanart Wanitchakoolb, Pakit Kumboonmac, Nutnaree Srisarakornb, Premyuda Hempoomb, Chatchai Muanprasartd
ABSTRACT: Colorectal cancer remains a leading cause of cancer-related mortality globally. Apoptosis induction is
a crucial target for developing effective chemotherapeutics. [6]-shogaol, a ginger-derived compound, has demonstrated anticancer potential by triggering apoptosis in various cancer types. This study investigated the apoptosisand autophagy-inducing and antiproliferative effects of 4F-phenyl pyrazole, a synthetic [6]-shogaol derivative, in
colorectal adenocarcinoma cells. Our findings demonstrated that 4F-phenyl pyrazole effectively reduced colorectal
adenocarcinoma HT29 cell viability, showing comparable efficacy to [6]-shogaol with an IC50 of 9.7 ?M. It also
significantly inhibited colony formation, indicating its antiproliferative properties. Mechanistic studies revealed dosedependent increases in phosphatidylserine exposure and anoctamin6 (ANO6) expression at 10, 20, and 40 ?M after
72 h of 4F-phenyl pyrazole treatment, suggesting apoptosis induction. Similar dose-dependent effects were observed
for LC3 expression, a marker of autophagy. Furthermore, 40 ?M 4F-phenyl pyrazole treatment resulted in diminished
spheroid formation. These results suggest that 4F-phenyl pyrazole is a promising candidate for further development as
an anticancer agent. Further investigations into its therapeutic potential and underlying mechanisms are warranted.
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| a |
Department of Applied Biology, Faculty of Science and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima 30000 Thailand |
| b |
Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170 Thailand |
| c |
Department of Applied Chemistry, Faculty of Science and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima 30000 Thailand |
| d |
Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samut Prakan 10540 Thailand |
* Corresponding author, E-mail: lalida.si@rmuti.ac.th
Received 8 Jul 2024, Accepted 25 Oct 2024
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