Research articles
ScienceAsia 50 (2024):ID 2024085 1-12 |doi:
10.2306/scienceasia1513-1874.2024.085
Betulinic acid inhibits proliferation and triggers apoptosis in
human breast cancer cells by modulating ER (α/β) and p53
Yanvit Prompoona, Laphatrada Yurasakponga, Athikhun Suwannakhana, Chidchanok Chawiwithayaa, Charoonroj Chotwiwatthanakunb, Wattana Weerachatyanukula, Chanin Nantasenamatc, Somluk Asuvapongpatanaa,*
ABSTRACT: Although betulinic acid (BA) has been shown to attenuate breast cancer cell lines, owing to its interaction
with several signaling molecules; its potential interaction with estrogen receptors (ERs) and p53 is not fully understood.
Hence, we aimed to investigate the anti-cancer effect of BA on breast cancer cells, focusing on its molecular mechanisms
involving the ER and p53 signaling pathways. The cell cytotoxicity of ER-positive (MCF-7) and ER-positive (MDA-MB231) breast cancer cells was studied using MTT assay. Apoptosis was investigated by flow cytometry and Western
blot analysis. The expression levels of ER?/ER? and wt-p53/mu-p53 were studied using Western blotting. Finally, a
possible interaction between BA and its molecular targets was predicted using molecular docking. Upon BA treatment,
both breast cancer cell lines underwent significant cell death and inhibition of cell proliferation. Flow cytometry and
Western blot analysis showed that the MCF-7 cells underwent early and late apoptosis, while MDA-MB-231 underwent
both apoptosis and necrosis within 48 h. The expression levels of ER?/ER? and wt-p53/mu-p53 were significantly
altered. This could be partly attributed to the activation of apoptosis and inhibition of proliferation through the p53
signaling pathway, as induced by the interaction of BA with its coupling molecules.
Download PDF
5 Downloads 101 Views
a |
Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400 Thailand |
b |
Academic and Curriculum Division, Nakhon Sawan Campus, Mahidol University, Nakhon Sawan 60130 Thailand |
c |
Streamlit Open Source, Snowflake Inc., San Mateo, CA 94402 USA |
* Corresponding author, E-mail: somluk.asu@mahidol.ac.th
Received 16 Jul 2023, Accepted 28 May 2024
|