Research articles
ScienceAsia 51 (2023): 1-9 |doi:
10.2306/scienceasia1513-1874.2023.014
Trace and qualitative evaluation of residual piperazine in chicken muscle by HPLC-MS/MS employing automatic ASE coupled to solid-phase preconcentration
Pengfei Gaoa,b, Yawen Guoa,b, Zhaoyuan Hea,b, Mengxiao Xuea, Yuhao Dongc, Kaizhou Xiea,b,*
ABSTRACT: This paper described a clipping, reliable, and hypersensitive protocol for the trace measurement of
piperazine residues in chicken muscle by high-performance liquid chromatography-tandem mass spectrometry (HPLC
MS/MS). The sample matrices were defatted with n-hexane and extracted with 2% trichloroacetic acid/acetonitrile
using accelerated solvent extraction (ASE) at 80?C and 1500 psi. Before using the HPLC system for separation, the
samples were purified utilizing solid-phase extraction (SPE) and filtered with a 0.22-?m syringe. The qualitative and
quantitative analyses applied electrospray ionization positive ion (ESI+) mode andmultiplereactionmonitoring(MRM)
mode combined with an external standard method. Under optimized sample pretreatment measures and analytical
conditions, the test results exhibited good linearity over the added concentration range (1?200 ?g/kg). When the
target was added at concentrations of 50, 100, and 200 ?g/kg, the recoveries were 82.22%?88.63%, and the relative
standard deviations (RSDs) were 1.56%?4.55%. The limit of detection (LOD) and the limit of quantification (LOQ)
were 0.3 ?g/kg and 1.0 ?g/kg, respectively. This method met the requirements of the EU and was successfully applied
for the determination of piperazine residues in chicken muscle samples.
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| a |
Department of Animal Science and Technology, Yangzhou University, Yangzhou 225009 China |
| b |
Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009 China |
| c |
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095 China |
* Corresponding author, E-mail: yzxkz168@163.com
Received 22 Sep 2023, Accepted 31 Dec 2024
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