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Short Report
ScienceAsia 32 (2006): 421-427 |doi: 10.2306/scienceasia1513-1874.2006.32.421
Identification of the Polyhedrin Gene of
Thai Bombyx mori Nucleopolyhedrovirus
Mallika Kaewwises1,2, Sudawan Chaeychomsri3 , Srimek Chowpongpang4 and Tipvadee
Attathom5*
ABSTRACT:The full-length of the polyhedrin gene (polh) of Thai Bombyx mori nucleopolyhedrovirus (BmNPV)
was cloned and sequenced. The polh sequence contained a 735 bp open reading frame (ORF) encoding a
protein of 245 amino acids with a predicted molecular mass of 28.8 kDa. The nucleotide sequence of Thai
BmNPV polh shows greater than 98% identity to the sequences of five different BmNPV polh genes that were
previously characterized. The high degree of sequence identity with the polh sequences of other BmNPVs
suggests that they are orthologues of the BmNPV polh gene in this study. Comparison of Thai BmNPV polh
sequence with other polhs of Lepidoteran NPVs (Autographa californica, Helicoverpa armigera, Spodoptera
litura and S. exigua) indicated that the nucleotide and amino acid sequences were greater than 65% and 78%
identical, respectively.
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1 Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140,
Thailand.
2 Biotechnology Research and Development Office, Sirindhon Plant Genetic Resources Building,
Thanyaburi, Pathum Thani 12110, Thailand.
3 Central Laboratory and Greenhouse Complex, Kasetsart University, Kamphaeng Saen, Nakhon Pathom
73140, Thailand.
4 Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Chatuchak, Bangkok 10900,
Thailand.
5 Department of Entomology, Faculty of Agriculture Kamphaeng Saen, Kasetsart University, Kamphaeng
Saen, Nakhon Pathom 73140, Thailand.
* Corresponding author, E-mail: agrtva@ku.ac.th
Received 19 Aug 2005,
Accepted 5 Jun 2006
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