Research articles
ScienceAsia 52 (2026): 1-8 |doi:
10.2306/scienceasia1513-1874.2026.077
Prunus avium lyophilized extract ameliorates renal oxidative
injury: in cellulo and in vivo study
Hattan A. Alharbia, Syed Raftullaha, Tanzeel Ahmedb,*, Tanveer Yaqubc, Mohammed S. Al-Dosaria,
Mohammed A. Hawwala, Nemat Alid, Mohammad K. Parveza,*
ABSTRACT: Prunus avium (sweet cherry) fruits are rich in bioactive phytochemicals that exhibit potent antioxidant
properties, as well as anti-inflammatory, anti-tumor, hypoglycemic, and anti-obesity activities. In this study, first,
the lyophilized sweet cherry extract (LCE; 250 mg/ml) showed in vitro antioxidant potential comparable to that
of ascorbic acid and rutin in DPPH free-radical scavenging and ?-carotene-linoleic acid bleaching assays. Further,
in cellulo assessment of LCE (200 g/ml) showed restoration of cell viability in HEK293 (transformed kidney cells)
and HUVEC (primary umbilical endothelial cells) by ?19% (p < 0.01) by attenuating oxidative stress and reducing
apoptotic cell death via caspase-3/7 activity by ?18% against DCF-induced cytotoxicity. Further, in the CCl4-treated
rats, LCE (500 mg/kg) significantly lowered the serum levels of urea (p < 0.001), uric acid (p < 0.001), and creatinine
(p < 0.001) to levels close to those observed with silymarin (standard) treatment. In addition, LCE significantly
reduced renal malondialdehyde level and increased nonprotein sulfhydryl and total protein contents, indicating its
in vivo antioxidative effects. Moreover, LCE also improved CCl4-induced histological lesions by reducing necrosis
and inflammation in kidney architecture. Further, the quantitative HPTLC validation showed presence of antioxidant
phytoconstituents, ?-sitosterol (1.45 g/mg) and ?-amyrin (0.61 g/mg), in the extract. In conclusion, sweet cherry
fruit significantly and potently protected against chemical-induced renal oxidative injury in both cell culture and a rat
model, attributed to its antioxidant constituents.
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| a |
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451 Saudi Arabia |
| b |
School of Biotechnology, IFTM University, Moradabad 244102 India |
| c |
Faculty of Medicine, Charles University, Hradec Kralove 50003, Czech Republic |
| d |
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451
Saudi Arabia |
* Corresponding author, E-mail: ahmed.tanzeel@gmail.com, mohkhalid@ksu.edu.sa
Received 10 Apr 2026, Accepted 1 Aug 2026
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