| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 50 Number 1
Volume 49 Number 6
Volume 49 Number 5
Volume 49S Number 1
Volume 49 Number 4
Volume 49 Number 3
Earlier issues
Volume  Number 

next article

Research articles

ScienceAsia 43 (2017): 145-154 |doi: 10.2306/scienceasia1513-1874.2017.43.145


Varietal verification of Thai rice using ultrathin layer isoelectric focusing of seed proteins


PakananÿJiamtaea, DamrongvudhiÿOnwimola,*, WanchaiÿChanpraserta, ThammasakÿThongketb, AnchareeÿPrasertsakc

 
ABSTRACT:     A suitable extraction solvent of seed protein for the identification of Thai rice varieties was determined for use in ultrathin layer isoelectric focusing (UTLIEF). Breeder seeds of 16 varieties were used and 3 extraction buffers, namely, phosphate buffer, NaCl, and water (with gel pHÿ2–9) were compared. The most suitable extraction buffer was phosphate buffer, which gave the highest efficacy of identification (98%) compared to NaCl (90%) and water (89%). The phosphate buffer was then tested in varietal verification using examination of individual seeds and testing bulk samples. The results show that the method of examination of individual seeds could clearly differentiate the contaminant variety from the tested variety, while testing bulk samples (10 seeds) could identify the contaminant variety when the ratio of seed number of the contaminant variety to the tested variety was 4–6 and higher. It can be concluded that phosphate buffer is suitable to be used in UTLIEF which is a promising method for varietal verification of Thai rice.

Download PDF

238 Downloads 1978 Views


a DepartmentÿofÿAgronomy, FacultyÿofÿAgriculture, KasetsartÿUniversity, Bangkokÿ10900ÿThailand
b DepartmentÿofÿHorticulture, FacultyÿofÿAgricultureÿatÿKamphaeng-Saen, KasetsartÿUniversity, NakhonÿPathomÿ73140ÿThailand
c BureauÿofÿRiceÿResearchÿandÿDevelopment, RiceÿDepartment, Bangkokÿ10900ÿThailand

* Corresponding author, E-mail: damrongvudhi.o@ku.th

Received 13 Jan 2016, Accepted 28 Jun 2017