Research articles
ScienceAsia 38 (2012): 44-53 |doi:
10.2306/scienceasia1513-1874.2012.38.044
Identification of Acetobacter strains isolated in Thailand based on the phenotypic, chemotaxonomic, and molecular characterizations
Jintana Kommaneea, Somboon Tanasupawata,*, Pattaraporn Yukphanb, Nuttha Thongchulc, Duangtip Moonmangmeed, Yuzo Yamadab,e
ABSTRACT: The restriction analysis of 16S-23S rRNA gene internal transcribed spacer regions (ITS) using TaqI, AluI, HpaII, and AvaII revealed that forty-seven bacterial isolates found in fruits and flowers collected in Thailand belong to the genus Acetobacter. They were identified as follows: Group 1 containing 11 isolates was identified as A. pasteurianus, Group 2 containing nine isolates was identified as A. orientalis, Group 3 containing eight isolates was identified as A. lovaniensis, Group 4 including eight isolates was identified as A. indonesiensis, Group 5 containing three isolates was identified as A. tropicalis, Group 6 containing five isolates was identified as A. ghanensis, and Group 7 containing four isolates was identified as A. orleanensis. The differentiation of Acetobacter species by the 16S-23S rRNA gene ITS restriction analysis was discussed. The phenotypic, chemotaxonomic, and molecular characteristics including 16S rRNA gene sequences supported the above-mentioned identification. All Thai isolates were screened for their alcohol dehydrogenase activity and exhibited an activity of 2.05–7.52 units/mg protein at 30 °C. Isolate PHD-23 produced the highest quantity of 1.50% acetic acid (v/v) from 4.0% ethanol (v/v).
Download PDF
4 Downloads 1149 Views
a |
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330 Thailand |
b |
BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani 12120 Thailand |
c |
The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330 Thailand |
d |
Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok 10140 Thailand |
e |
JICA Senior Overseas Volunteer, Japan International Cooperation Agency (JICA), Shibuya-ku, Tokyo 151-8558, Japan, Professor Emeritus, Shizuoka University, Shizuoka 422-8529, Japan |
* Corresponding author, E-mail: Somboon.T@chula.ac.th
Received 14 Sep 2011, Accepted 7 Dec 2011
|