| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 50 Number 1
Volume 49 Number 6
Volume 49 Number 5
Volume 49S Number 1
Volume 49 Number 4
Volume 49 Number 3
Earlier issues
Volume  Number 

previous article next article

Research articles

ScienceAsia 38 (2012): 44-53 |doi: 10.2306/scienceasia1513-1874.2012.38.044


Identification of Acetobacter strains isolated in Thailand based on the phenotypic, chemotaxonomic, and molecular characterizations


Jintana Kommaneea, Somboon Tanasupawata,*, Pattaraporn Yukphanb, Nuttha Thongchulc, Duangtip Moonmangmeed, Yuzo Yamadab,e

 
ABSTRACT:     The restriction analysis of 16S-23S rRNA gene internal transcribed spacer regions (ITS) using TaqI, AluI, HpaII, and AvaII revealed that forty-seven bacterial isolates found in fruits and flowers collected in Thailand belong to the genus Acetobacter. They were identified as follows: Group 1 containing 11 isolates was identified as A. pasteurianus, Group 2 containing nine isolates was identified as A. orientalis, Group 3 containing eight isolates was identified as A. lovaniensis, Group 4 including eight isolates was identified as A. indonesiensis, Group 5 containing three isolates was identified as A. tropicalis, Group 6 containing five isolates was identified as A. ghanensis, and Group 7 containing four isolates was identified as A. orleanensis. The differentiation of Acetobacter species by the 16S-23S rRNA gene ITS restriction analysis was discussed. The phenotypic, chemotaxonomic, and molecular characteristics including 16S rRNA gene sequences supported the above-mentioned identification. All Thai isolates were screened for their alcohol dehydrogenase activity and exhibited an activity of 2.05–7.52 units/mg protein at 30 °C. Isolate PHD-23 produced the highest quantity of 1.50% acetic acid (v/v) from 4.0% ethanol (v/v).

Download PDF

4 Downloads 1149 Views


a Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330 Thailand
b BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani 12120 Thailand
c The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330 Thailand
d Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok 10140 Thailand
e JICA Senior Overseas Volunteer, Japan International Cooperation Agency (JICA), Shibuya-ku, Tokyo 151-8558, Japan, Professor Emeritus, Shizuoka University, Shizuoka 422-8529, Japan

* Corresponding author, E-mail: Somboon.T@chula.ac.th

Received 14 Sep 2011, Accepted 7 Dec 2011